The following strains were used in this study: Staphylococcus aureus ATCC 6538, biofilm-producing reference strain [58 (link)], S. aureus ATCC 43300 (Methicillin resistant S. aureus-MRSA), Pseudomonas aeruginosa ATCC 9027, P. aeruginosa DSM 102273 (multidrug resistant). The strains were stored in the private collection of the Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina (Messina, Italy). They were stored at −70 °C in Microbanks™ (Pro-lab Diagnostics, Neston, UK).
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