Cellular proliferation was analyzed with the MTT assay as previously described (49 (link)). To determine the effect of p38 MAPK inhibition on the proliferation of MCF7 cells, we cultured cells in 24-well plates overnight and then treated them with 10 μM SB203580 in the presence of 1% FBS for various times (1 to 3 days) followed by incubation with MTT solution for 2 hours at 37°C. After removing the media, we dissolved crystals of MTT formazan in DMSO and measured the samples with a Bio-Rad plate reader at a wavelength of 560 nm.
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Measuring Cell Proliferation with MTT Assay
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Corresponding Organization :
Other organizations : Augusta University, Fudan University, University of Toledo Medical Center, Shanghai University of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Augusta University Health
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Variable analysis
independent variables
- Concentration of SB203580 (10 μM)
- Cellular proliferation (measured by MTT assay)
- Incubation time (1 to 3 days)
- Cell line (MCF7)
- Serum concentration (1% FBS)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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