To detect anti-SARS-CoV-2 antibodies, we used two commercially available immunoassays: the Roche Elecsys anti-SARS-CoV-2 S and anti-SARS-CoV-2 N immunoassays (Roche Diagnostics, Rotkreuz, Switzerland), which detect immunoglobulins (IgG/A/M) against the receptor binding domain of the virus spike (S) protein (#09 289 275 190, Roche-S) and the virus nucleocapsid (N) protein (#09 203 079 190, Roche-N), respectively. Both assays have high accuracy and have been validated in multiple settings, including our previous serosurveys.20 , 21 (link), 22 (link) We defined seropositivity using the manufacturer's provided cut-off values of titer ≥0.8 U/mL for the Roche-S, and cut-off index ≥1.0 for the Roche-N immunoassays.
Other organizations :
University Hospital of Geneva, Johns Hopkins University, University of Lausanne, École Polytechnique Fédérale de Lausanne, University of Geneva, World Health Organization
Cut-off values for seropositivity: ≥0.8 U/mL for Roche-S, ≥1.0 cut-off index for Roche-N
positive controls
Not explicitly mentioned
negative controls
Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
