A total of 794 serum samples were randomly selected to detect cytokines. A total of 27 cytokines were measured using the Bio-Plex Pro Human Cytokine 27-plex assay (Bio-Rad, M500KCAF0Y)22 (link) and Luminex 200 platform (Luminex Corporation, Austin, TX, USA). The assays included a series of known concentrations to generate standard curves constructed by Bio-Plex Manager 6.0 (Bio-Rad Laboratories). For samples with values below the assay’s lower limit of detection, values equal to half of the lower limit of detection were used.23 (link)
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Multiplex Cytokine Profiling in Serum
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Other organizations : Sun Yat-sen University, Sun Yat-sen Memorial Hospital, The First Affiliated Hospital, Sun Yat-sen University, Third Affiliated Hospital of Sun Yat-sen University
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- Concentration of 27 cytokines measured using the Bio-Plex Pro Human Cytokine 27-plex assay (Bio-Rad, M500KCAF0Y) and Luminex 200 platform (Luminex Corporation, Austin, TX, USA)
- A total of 794 serum samples were randomly selected
- A series of known concentrations were used to generate standard curves constructed by Bio-Plex Manager 6.0 (Bio-Rad Laboratories)
- For samples with values below the assay's lower limit of detection, values equal to half of the lower limit of detection were used
- Not specified
- Not specified
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