Muscle Biopsy and Tissue Analysis

Muscle biopsies from PRE and POST testing sessions were collected using a five gauge needle under local anesthesia as previously described (Mobley et al., 2017 (link)). Immediately following tissue procurement, ∼20–40 mg of tissue was embedded in cryomolds containing optimal cutting temperature media (Tissue-Tek^®; Sakura Finetek Inc, Torrence, CA, USA) for fCSA assessment. The remaining tissue was teased of blood and connective tissue, wrapped in pre-labelled foils, flash frozen in liquid nitrogen (LN₂), and subsequently stored at −80 °C until protein and citrate synthase activity analyses described below.

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Roberts M.D., Romero M.A., Mobley C.B., Mumford P.W., Roberson P.A., Haun C.T., Vann C.G., Osburn S.C., Holmes H.H., Greer R.A., Lockwood C.M., Parry H.A, & Kavazis A.N. (2018). Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training. PeerJ, 6, e5338.

Publication 2018

Tissue-Tek

Biopsies Blood Citrate synthase Connective tissue Frozen Local anesthesia Muscle Needle Nitrogen Protein Tissue Tissue procurement

Corresponding Organization :

Other organizations : Auburn University, Draper Laboratory

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Variable analysis

independent variables

Muscle biopsies from PRE and POST testing sessions

dependent variables

Fiber cross-sectional area (fCSA) assessment
Protein analysis
Citrate synthase activity

control variables

Local anesthesia was used for muscle biopsies
Muscle tissue was immediately frozen in liquid nitrogen and stored at -80°C

controls

No positive or negative controls were explicitly mentioned in the input.

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