Circulating activated platelet level was evaluated ex vivo by activated platelet ability to form so-called platelet-leukocyte complexes (PLC). Whole-blood PLC were counted by flow cytometry [15 (link)]. The samples were analyzed with a FACScalibur device (Becton-Dickinson, Le Pont de Claix, France). PLC were identified as the cell population stained with both anti-CD41-PE (Beckman Coulter) and anti-CD45-FITC (Beckman Coulter) and expressed as a percentage of total leukocytes.
Circulating activated platelet level evaluated ex vivo by activated platelet ability to form platelet-leukocyte complexes (PLC)
control variables
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controls
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Negative control: None mentioned
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