Proteomic analysis was performed as previously described23 (link). Briefly, purified particles were denatured with heat and loaded onto a 15% (w/v) polyacrylamide gel. Proteins were stained with Coomassie Brilliant Blue R250 dye and washed with methanol–acetic acid–H2O.
To identify phage structural proteins, we performed the above mentioned experiment for 30 min. The protein band that included all the structural proteins was excised from the gel for HPLC-MS analysis. HPLC-MS data were processed by the Agilent Spectrum Mill proteomics software to allocate each protein to the corresponding gene.
To identify phage structural proteins, we performed the above mentioned experiment for 30 min. The protein band that included all the structural proteins was excised from the gel for HPLC-MS analysis. HPLC-MS data were processed by the Agilent Spectrum Mill proteomics software to allocate each protein to the corresponding gene.
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