Enzyme activity was quantified by fluorescence kit using a specific anti-MMP-9 monoclonal antibody and a fluorogenic substrate (SensoLyte Plus 520 MMP-9 Assay Kit; AnaSpec, Inc., Fremont, CA, USA), as described previously.29 (link) MMP-9 induced cleavage of the fluorogenic peptide was measured at 490 nm excitation and 520 nm emission wavelengths.
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Quantifying MMP-9 Activity Using Fluorescent Assay
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Anti antibody
Assay
Cleavage
Enzyme activity
Fluorescence
Fluorogenic substrate
Mmp 9
Monoclonal antibody
Peptide
Corresponding Organization : Wayne State University
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Variable analysis
independent variables
- Enzyme activity
- MMP-9 induced cleavage of the fluorogenic peptide measured at 490 nm excitation and 520 nm emission wavelengths
- Specific anti-MMP-9 monoclonal antibody
- Fluorogenic substrate (SensoLyte Plus 520 MMP-9 Assay Kit)
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