All strains were derived from Escherichia coli K12 strain NCM3722 (Soupene et al, 2003; Lyons et al, 2011) as listed in Supplementary Table 1 and detailed in Supplementary Methods . MOPS-buffered minimal media (pH 7.4) were used for cell growth as described by Neidhardt et al (1974). For carbon sources, glycerol (0.4% w/v), glucose (0.4% w/v), or 10 mM each of glucose-6 phosphate and gluconate were used. For nitrogen sources, different concentrations of NH4Cl were used as specified. All the media were filtered through 0.45 μm filters.
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E. coli Strain Characterization
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Other organizations : University of California, San Diego, Indiana University – Purdue University Indianapolis, Center for Theoretical Biological Physics
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Variable analysis
independent variables
- Carbon sources: glycerol (0.4% w/v), glucose (0.4% w/v), or 10 mM each of glucose-6 phosphate and gluconate
- Nitrogen sources: different concentrations of NH4Cl
- Not explicitly mentioned
- Escherichia coli K12 strain NCM3722 as the base strain
- MOPS-buffered minimal media (pH 7.4) for cell growth
- All media were filtered through 0.45 μm filters
- Positive control: Not mentioned
- Negative control: Not mentioned
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