In Vitro and In Vivo Evaluation of VS-5584 and ABT-737

VS-5584 and ABT-737 were obtained from Selleck (Shanghai, China). Both compounds were dissolved in DMSO and stored at -20° for in vitro studies. For in vivo studies, both agents were dissolved in the SX-1292 oral vehicle [1% sodium carboxymethyl cellulose, 0.5% sodium lauryl sulfate (SLS), and 0.05% antifoam; Eli Lilly, Shanghai, China] and administered by gastric lavage [13 (link),14 (link)]. Antibodies of p-AKT (Ser 473, #9271), p-AKT (Thr 308, #9275), p-S6 ribosomal protein (S6, Ser 235/236, #2211), S6 (#2317), p-p70 S6 kinase 1 (S6K1, Thr 398, #9209), S6K1 (#2708), Bcl-2 (#2870), Bcl-xL (#2762) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, #2118) were obtained from Cell Signaling Technology (Danvers, MA). The concentration of primary antibodies utilized in this study was 1: 1,000, except for p-S6 (1: 10,000) and GAPDH (1: 10,000). The caspase inhibitors z-VAD-fmk and z-DVED-fmk were obtained from Calbiochem (Shanghai, China).