The experiments were performed according to the previous report [9 (link)]. Proteins were quantified using a BCA protein assay kit (Thermo Scientific, Waltham, MA), and 25 μg proteins were separated using 10% SDS-PAGE and transferred to a PVDF membrane (Millipore). The antibodies for USP5 (1 : 500, #ab155993), TRAF6 (1 : 500, #ab33915), p65 (1 : 500, #ab16502), phos-p65 (1 : 500, #ab76302), IκBα (1 : 500, #ab32518), phos-IκBα (1 : 500, #ab133462), β-actin (1 : 1000, #ab179467), Myc (1: 1000, #ab32), Flag (1 : 1000, #ab49763), and K48-ub (1 : 500, #ab140601) were all bought from Abcam (Abcam, Cambridge, USA).
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