Murine Immune Cell Lineages Characterization

C57BL/6 CD45.2⁺ WT mice were purchased from Taconic Biosciences (Rensselaer, NY, USA) or Japan SLC (Hamamatsu, Japan). Rag2 KO and CD45.1⁺ WT mice were obtained from the National Institute of Allergy and Infectious Diseases (NIAID) contract facility at Taconic Biosciences or breeding stock maintained at Tohoku University Graduate School of Medicine. Nur77-GFP and Foxp3-RFP reporter mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA). CD4-CreERT2 TCRα^flox mice are previously described (5 (link)). All mice were maintained in SPF animal facilities in the NIAID, National Cancer Institute (NCI), National Institutes of Health (NIH), or Tohoku University Graduate School of Medicine except for GF and AF mice, which were bred and maintained in the animal facility of Pohang University of Science and Technology as previously described (33 (link)). All mice were used at the age of 8–16 weeks except in the case of Figures 3A, B, where mice of indicated ages were utilized. The care and handling of the animals used in our studies were in accordance with the animal study protocols approved by the NIAID or NCI Animal Care and Use Committee, by the Institutional Committee for the Use and Care of Laboratory Animals of Tohoku University, or by the Institutional Animal Care and Use Committees of the Pohang University of Science and Technology.