Testosterone and Estrogen Measurement Protocol

Serum levels of testosterone and estrogen were measured according to the manufacturer. Testosterone levels were measured using the Testosterone ELISA kit (#29K116 42K034DRG Instruments GmbH, Germany). The assay detection limit for testosterone was 0.083 ng/mL. The intra-assay coefficient of variation for each assay was between 3.28% and 4.16%. The inter-assay coefficient of variation for each assay was between 4.73% and 9.94%. Estrogen levels were measured using the Estrogen ELISA kit (#42K026-3DRG Instruments GmbH,Germany) (25 (link), 26 (link)). The assay detection limit for estradiol was 10.60 pg/mL. The intra-assay coefficient of variation for each assay was between 8.70% and 9.23%. The inter-assay coefficient of variation for each assay was between 6.87% and 14.91%.

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do Val Lima P.R., Ronconi K.S., Morra E.A., Rodrigues P.L., Ávila R.A., Merlo E., Graceli J.B., Simões M.R., Stefanon I, & Ribeiro Júnior R.F. (2023). Testosterone deficiency impairs cardiac interfibrillar mitochondrial function and myocardial contractility while inducing oxidative stress. Frontiers in Endocrinology, 14, 1206387.

Publication 2023

Testosterone ELISA kit

Assay Elisa Estradiol Estrogen Serum Testosterone

Corresponding Organization :

Other organizations : Universidade Federal do Espírito Santo

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum levels of testosterone
Serum levels of estrogen

control variables

None explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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