Detergent-insoluble extract fractions were digested with modified trypsin (Promega) using the FASP protocol [59 (link)]. Equal amounts of each peptide sample were labeled using the 10-plex TMT Reagents (Thermo Fisher Scientific) and then analyzed via liquid chromatography-mass spectrometry (LC-MS/MS) [60 (link),61 (link),62 (link),63 (link)].
Free full text: Click here