The adhesion ability of the bacteria was measured in HT-29 cells and hPSC-derived hIECs. The cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Cytiva, USA) with 10% fetal bovine serum (FBS; HyClone, USA) and 1% penicillin/streptomycin (PS; Gibco, USA) at 37°C in a humidified incubator with 5% CO2.
The hPSC line (H9) was purchased from the WiCell Research Institute (Madison, USA). The hPSCs were cultured on Mitomycin C-treated mouse embryonic fibroblasts (MMC-MEF) using hPSC medium [Dulbecco’s modified Eagle’s medium (DMEM)/F12 (Gibco), 10% serum replacement (SR, Gibco), 1% PS (Gibco), 1%GlutaMAX (Gibco), 0.1% β-mercaptoethanol (Gibco), and 8 ng/ml basic fibroblast growth factor (bFGF; R&D Systems, USA)]. The hPSCs were passaged every week to newly prepared MMC-MEF [19 (link)]. All experiments were approved by the Public Institutional Review Board designated by the Ministry of Health and Welfare (P01-201409-ES-01).
The hPSC line (H9) was purchased from the WiCell Research Institute (Madison, USA). The hPSCs were cultured on Mitomycin C-treated mouse embryonic fibroblasts (MMC-MEF) using hPSC medium [Dulbecco’s modified Eagle’s medium (DMEM)/F12 (Gibco), 10% serum replacement (SR, Gibco), 1% PS (Gibco), 1%GlutaMAX (Gibco), 0.1% β-mercaptoethanol (Gibco), and 8 ng/ml basic fibroblast growth factor (bFGF; R&D Systems, USA)]. The hPSCs were passaged every week to newly prepared MMC-MEF [19 (link)]. All experiments were approved by the Public Institutional Review Board designated by the Ministry of Health and Welfare (P01-201409-ES-01).
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