Detecting and Characterizing Antibiotic Resistance Genes

The major acquired extended-spectrum β-lactamase (Dallenne et al., 2010 (link); Hussain et al., 2014 (link); Ranjan et al., 2017 (link)), fosfomycin (Dantas Palmeira et al., 2018 (link)), chloramphenicol (White et al., 1999 (link)), lincosamide (Garcia-Martin et al., 2018 (link)), and carbapenemase genes (Chen et al., 2015 (link); Ranjan et al., 2016 (link), 2017 (link)) were detected by PCR, after which all the PCR amplicons were sequenced on the ABI 3730 platform (Applied Biosystems, United States). The sodium azide-resistant E. coli J53Azi^R strain was used as the recipient and the P. mirabilis HFK418 isolate as the donor for the conjugative transfer of the plasmids. The conjugal transfer tests were performed as described previously (Srijan et al., 2018 (link)), and the conjugation frequency was calculated as transconjugants divided by number of donors.

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Dong D., Li M., Liu Z., Feng J., Jia N., Zhao H., Zhao B., Zhou T., Zhang X., Tong Y, & Zhu Y. (2019). Characterization of a NDM-1- Encoding Plasmid pHFK418-NDM From a Clinical Proteus mirabilis Isolate Harboring Two Novel Transposons, Tn6624 and Tn6625. Frontiers in Microbiology, 10, 2030.

Publication 2019

ABI 3730 platform

Carbapenemase Chloramphenicol Donors E coli Fosfomycin Genes Lincosamide Mirabilis Plasmids Sodium azide Strain β lactamase

Corresponding Organization : Beijing University of Chemical Technology

Other organizations : Hebei Normal University

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Variable analysis

independent variables

Conjugative transfer of plasmids from the *P. mirabilis* HFK418 isolate (donor) to the sodium azide-resistant *E. coli* J53Azi^R strain (recipient)

dependent variables

Conjugation frequency, calculated as transconjugants divided by number of donors

control variables

The conjugal transfer tests were performed as described previously (Srijan et al., 2018)

controls

The sodium azide-resistant *E. coli* J53Azi^R strain was used as the recipient
No negative controls were explicitly mentioned in the input protocol.

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