Embryos were obtained through natural matings of heterozygous transgenic CAG-TAG1 males (Trichas et al., 2008 (link)) with wild-type CD1 females (Charles River). Noon of the day when the vaginal plug was found was considered 0.5 days post coitum (dpc). Embryos were isolated from the oviduct at 2.5 dpc in M2 media (Sigma) and then held in KSOM (Millipore catalogue number MR-020P-5F) before setting up for culture.
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