Indirect Immunofluorescence Staining Protocol

Indirect immunofluorescence staining was performed as described in previous studies (Oppel et al., 2019 (link), 2011 (link); Ball et al., 2017 (link)). Primary antibodies used were pan-cytokeratin (AE1/AE3; Novus Biologicals #NBP2-29429; 1:200) and tri-methyl-Histone H3 (Lys27) (C36B11; Cell Signaling Technology #9733; 1:400). Secondary antibodies used were goat anti-rabbit-IgG and goat anti-mouse-IgG conjugated with Alexa 488 or 568 (Life Technologies; 1:400).

Free full text: Click here

Oppel F., Ki D.H., Zimmerman M.W., Ross K.N., Tao T., Shi H., He S., Aster J.C, & Look A.T. (2020). suz12 inactivation in p53- and nf1-deficient zebrafish accelerates the onset of malignant peripheral nerve sheath tumors and expands the spectrum of tumor types. Disease Models & Mechanisms, 13(8), dmm042341.

Publication 2020

tri-methyl-Histone H3 (Lys27) (C36B11; goat anti-mouse-IgG Alexa 488 or 568

Anti igg Antibodies Biologicals Cytokeratin Goat Histone h3 Indirect immunofluorescence Mouse Novus Rabbit

Corresponding Organization :

Other organizations : Dana-Farber Cancer Institute, Harvard University, Brigham and Women's Hospital

Top 5 similar protocols

Variable analysis

independent variables

Primary antibodies used: pan-cytokeratin (AE1/AE3) and tri-methyl-Histone H3 (Lys27) (C36B11)

dependent variables

Indirect immunofluorescence staining

control variables

Indirect immunofluorescence staining was performed as described in previous studies (Oppel et al., 2019, 2011; Ball et al., 2017)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!