NTA was applied to identify whether pellets were exosomes by detecting the particle size. Firstly, pellets were washed using deionized water. Then, polystyrene microspheres were applied to calibrate ZetaView analyzer (Particle Metrix, Meerbusch, Germany). Next, pellets were diluted with 1X PBS buffer (Biological Industries, Israel). After that, ZetaView analyzer was conducted to detect whether these pellets conformed to the structure and particle size of the exosomes according to the previous literature [40 (link),41 (link)].
Protocol detail
Exosome Characterization Using NTA
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Corresponding Organization : Hangzhou Medical College
Other organizations : Zhejiang University
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Variable analysis
independent variables
- Pellet washing using deionized water
- Dilution of pellets with 1X PBS buffer
- Particle size of pellets detected by ZetaView analyzer
- Conformity of pellets to the structure and particle size of exosomes according to previous literature
- Calibration of ZetaView analyzer using polystyrene microspheres
- Positive control: None specified
- Negative control: None specified
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