BLECs and pericytes were grown in endothelial cell medium (ECM) (Sciencell) supplemented with 5% fetal calf serum (Gibco), ECGS supplements, and 50 μg/ml gentamycin (Biological industries). In order to induce brain-like phenotype, ECs monolayers were grown in medium containing 2/3 ECM and 1/3 pericytes-conditioned medium (ECM medium collected after 3 days culture with pericytes), as previously published [26 (link), 27 (link)].
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