Genomic DNA of O. parapolymorpha IMD001 was isolated with the QIAGEN Genomic-tip 100/G kit (Qiagen, Hilden, Germany) from a stationary-phase overnight shake-flask culture grown on YPD medium, according to the manufacturer's instructions. Genomic DNA was quantified using a Qubit® 2.0 Fluorometer (Thermo Fisher Scientific). Ten micrograms of genomic DNA was sequenced by Novogene Bioinformatics Technology (Yuen Long, Hong Kong, China) on a HiSeq 2500 (Illumina, San Diego, CA, USA) with 150-bp paired-end reads using TruSeq PCR-free library preparation (Illumina). In order to verify complete absence of plasmid pUDP046, sequencing reads were mapped to the sequence of pUDP046 and to the genome of O. parapolymorpha CBS 11895 (accession GCF_000187245.1) (Ravin et al.2013 (link)) using the Burrows–Wheeler Alignment tool and further processed using SAMtools (Li et al.2009 (link); Li and Durbin 2010 (link)). The absence of sequences from pUDP046 was confirmed by visualizing the generated .bam files in the Integrative Genomics Viewer software (Robinson et al.2011 (link)). Sequencing data are available at NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA413643.