ChIP-seq for RPE1 cells was performed exactly as described [2 (link)], anti-RAD21 (Abcam, (Cambridge, UK); ab992; lot GR221348-8; dilution 1:150) and anti-CTCF (Active Motif (Waterloo Atrium, Drève Richelle 167–boîte 4, BE-1410 Waterloo, Belgium); 613111; lot 34614003; dilution 1:150) antibodies were used.
Peak calling was completed with the MACS2 pipeline [25 ]. Differential peak calling was completed with the DiffBind package using standard parameters [26 ].
To build heat maps for the RAD21 signal, we applied deepTools package [27 (link)], particularly, computeMatrix and plotHeatmap tools. H3K27ac tracks for the RPE1 cell line (GSM4194693) were used to map the RAD21 signal around acetylated regions.
Peak calling was completed with the MACS2 pipeline [25 ]. Differential peak calling was completed with the DiffBind package using standard parameters [26 ].
To build heat maps for the RAD21 signal, we applied deepTools package [27 (link)], particularly, computeMatrix and plotHeatmap tools. H3K27ac tracks for the RPE1 cell line (GSM4194693) were used to map the RAD21 signal around acetylated regions.
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