The intracellular ROS levels were determined by using a ROS Assay Kit (Beyotime, Shanghai, China) following the protocols and the methods described previously [29 (link)]. Target cells were treated under different conditions and incubated with 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) for thirty minutes at 37 °C. Then cells were harvested for flow cytometery (BD, USA) analysis [30 (link),31 (link)].
Free full text: Click here