Transient Transfection of Neuroblastoma Cells

We maintained human neuroblastoma SH-SY5Y cells (ATCC, Manassas, VA) in DMEM containing 10% FBS (v/v) and antibiotics (penicillin-streptomycin 100 U/mL, ThermoFisher Scientific). The cell culture incubator was set with a humidified atmosphere consisting of 5% CO2/95% air at 37 °C. To transiently transfect the indicated plasmids (pCMV-tTA, TetP-β23, pTet-dual2, and HA-α-synuclein [8 (link)]) to SH-SY5Y cells, the X-tremeGENE HP transfection reagents (Roche) were used following the manufacturer’s protocol.

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Ham S., Kim H., Yoon J.H., Kim H., Song B.R., Choi J.Y., Lee Y.S., Paek S.M., Maeng H.J, & Lee Y. (2019). Therapeutic Evaluation of Synthetic Peucedanocoumarin III in an Animal Model of Parkinson’s Disease. International Journal of Molecular Sciences, 20(21), 5481.

Publication 2019

SH-SY5Y cells penicillin-streptomycin X-tremeGENE HP transfection reagents

Antibiotics Atmosphere Cell culture Cells Human Neuroblastoma Penicillin Plasmids Streptomycin Transfection α synuclein

Corresponding Organization : Gyeongsang National University

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Variable analysis

independent variables

Plasmids transfected (pCMV-tTA, TetP-β23, pTet-dual2, and HA-α-synuclein)

dependent variables

Not explicitly mentioned

control variables

Cell type (human neuroblastoma SH-SY5Y cells)
Cell culture medium (DMEM containing 10% FBS and antibiotics)
Cell culture conditions (humidified atmosphere, 5% CO2/95% air, 37 °C)
Transfection reagent (X-tremeGENE HP)

controls

Positive control: Not specified
Negative control: Not specified

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