Fecal Biomarkers and Organic Acids

Fecal pH and organic acids were assessed at V1, V2, and V3 using pH-indicator paper (Merck, Darmstadt, Germany) and validated LCMS, respectively (23 (link)). ELISA kits were used to analyze fecal biomarkers at V1, V2, and V3 including secretory immunoglobulin A (sIgA), calprotectin (Immundiagnostik AG, Bensheim, Germany) and alpha-1-antitrypsin (AAT) (BioVendor – Laboratorni medicina a.s., Brno, Czech Republic). Fecal cytokines were quantified as previously published (24 (link)), diluted 1:2 in Meso Scale Discovery diluent (MSD; Rockville, MD, United States), using V-Plex Plus kits and U-plex according to manufacturer’s instructions, and a QuickPlex SQ 120 Imager (MSD; Rockville, MD, United States). Total protein content in fecal extracts was quantified using Pierce BCA protein assay kit (Thermo Scientific) and used for cytokine level normalization.

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Capeding M.R., Phee L.C., Ming C., Noti M., Vidal K., Le Carrou G., Frézal A., Moll J.M., Vogt J.K., Myers P.N., Nielsen B.H., Boulangé C.L., Samuel T.M., Berger B, & Cercamondi C.I. (2023). Safety, efficacy, and impact on gut microbial ecology of a Bifidobacterium longum subspecies infantis LMG11588 supplementation in healthy term infants: a randomized, double-blind, controlled trial in the Philippines. Frontiers in Nutrition, 10, 1319873.

Publication 2023

pH-indicator paper calprotectin QuickPlex SQ 120 Imager Pierce BCA protein assay kit

Corresponding Organization : Nestlé (Switzerland)

Other organizations : Asian Hospital and Medical Center, Clinical Microbiomics (Denmark)

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Variable analysis

dependent variables

Fecal pH
Fecal organic acids
Fecal secretory immunoglobulin A (sIgA)
Fecal calprotectin
Fecal alpha-1-antitrypsin (AAT)
Fecal cytokines

controls

None specified

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