Mice were anaesthetised and fundi imaged using an otoscope-based fiber-optic light device as described previously (9 (link)). In brief, anaesthesia was induced with an intraperitoneal injection of a mixture of 40 mg/kg Vetalar® (Fort Dodge Animal Health Ltd., Southampton, UK) and 0.2 - 1.0 mg/kg Domitor® (Orion Pharma, Espoo, Finland) diluted in injectable water. Pupils were dilated with Minims 1% (w/v) tropicamide, and 2.5% (w/v) phenylephrine hydrochloride (both from Chauvin Pharmaceuticals Ltd, UK). Viscotears Carbomer 2 mg/g liquid gel (Alcon Eyecare UK Ltd., Camberley, UK) was used throughout the procedure as lubrication to prevent corneal drying and lens opacification. Images of the central and peripheral retina were taken. EAU severity was evaluated separately in terms of inflammation and chorioretinal atrophy using a scoring system as previously described (9 (link), 34 (link)).
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