Western Blot, qRT-PCR, and Flow Cytometry

Western blot analysis was carried out as previously described 7 (link), 15 (link), 20 (link), 51 (link). Primary antibodies used are as follows: BRCA1, HSP90, GAPDH (Ambion), E-Cad, Vim, GATA3 (D13C9), (Cell Signaling), TWIST (Abcam). For qRT-PCR, total RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer's protocol and cDNA was generated using the Omniscript RT Kit (Qiagen). qRT-PCR was performed as previously reported 7 (link). For surface marker analysis, tumor cells were isolated and stained. After exclusion of lymphocytes, dead cells, and Lin+ cells, the expression profile for CD24 and CD29 was determined by flow cytometry as previously described 15 (link), 20 (link).