Evaluating GLP-1 Receptor Activation

The human GLP-1 receptor gene was introduced into CHO-K1 cells via transfection, following the methodology described in a recent study [35 (link)]. The effective introduction of genetic material was verified the next day using the quantitative polymerase chain reaction (qPCR) technique outlined below. Cells expressing GLP-1 receptors were then exposed to SA at the specified doses for 1 h. The intracellular cAMP levels were then assessed with a commercially available ELISA kit (ADI-900-067, Enzo Life Sciences, Farmingdale, NY, USA). The specified samples were subjected to triplicate assays. A control group of CHO-K1 cells that had not been transfected with the GLP-1 receptor gene was used. The cAMP concentrations of different groups were compared.