Characterization of Actin-related Protein Complex Mutants in Arabidopsis

Col-0 (WT), SALK_013909.27.65 (arpc4), SALK_123936.4 (arpc5), SALK_099449, SAIL_1210_A03C1 and SAIL_131_F01 (arpc3) T-DNA insertion mutant lines were obtained from The Nottingham Arabidopsis Stock Centre (NASC). The arpc4 (SALK_013909.27.65), arpc5 (SALK_123936.4) and arpc2 lines were already characterized in previous research produced in our laboratory^{25 (link),29 (link)} as were the complemented lines GFP:ARPC2 and ARPC5:mCherry³² . Plants were grown under a 16 h-light/8 h-dark cycle (light intensity 110 mmol/m²/s) at 22 °C either on peat pellets or in vitro. For in vitro cultures, seeds were briefly washed with 96% ethanol and then surface-sterilized by bleach solution (sodium hypochlorite solution at the final concentration of 2.5%) for ten minutes, they were sown on half-strength Murashige and Skoog (MS) medium (1% [w/v] sucrose, 2.2 g/l MS salts [Sigma/Aldrich], 0.8% agarose, [pH 5.7]).