Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membrane (Whatman). Membranes were washed and incubated with antibodies as described previously11 (link). Briefly, membranes were blocked with blocking buffer (Sigma-Aldrich) for 1 h and were incubated with appropriate primary antibodies diluted in blocking buffer overnight at 4 °C. After three 5-min washes, membranes were incubated with secondary antibodies diluted in blocking buffer for 45 min in the dark. Secondary antibodies used were donkey Alexa Fluor 680-conjugated anti-goat IgG or anti-mouse IgG (Life Technologies) and donkey IRDye 800-conjugated anti-mouse IgG (Rockland Immunochemicals). Membranes were washed in the dark and scanned using the Odyssey infrared imaging system (LI-COR).