Calcein labeling histomorphometric analysis was performed as reported previously.22 (link) Mice were intraperitoneally injected with Calcein (Sigma-Aldrich, USA) at 15 mg·kg−1 prepared in 2% sodium bicarbonate solution at 10 days and 3 days before sacrifice. Bone formation analyses using MAR and BFR were performed according to the standardized nomenclature for bone histomorphometry under a fluorescence microscope (IX71; Olympus, Japan).
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