The experiment was performed under alkaline conditions as previously described using the Single Cell Gel Electrophoresis Assay kit from Trevigen (cat # 4250-050-K, Gaithersburg, MD, USA). Fluorescent images were taken using the Cellsens software on a 10× objective of an Olympus IX70 microscope and analyzed by ImageJ (ImageJ 1.8.0, Bethesda, MD, USA) under the OpenComet plugin (http://cometbio.org/ , accessed on 17 August 2021) [37 (link)]. All images were taken with the same exposure time and magnification. Data were expressed by the tail DNA percentage (tail intensity out of total DNA intensity).
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