Stx expression was determined for filtered culture supernatants with or without mitomycin C induction, by using RIDASCREEN Kit Verotoxin enzyme immunoassay (R-Biopharm Latin America) and then semi-quantified, as previously described [20 (link)]. To measure Vero cell cytotoxicity, the strains were cultured overnight at 37°C in 5 ml of LB broth and the culture was centrifuged at 200 rpm. The supernatant was filtered (0.22 μm filters) and subsequently assayed for cytotoxicity on Vero cells, as previously described [20 (link)].
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