ChIP‐qPCR was performed as described in Poli et al (2016 (link)) using anti‐HA probe F‐7 (Santa Cruz, sc‐7392) and anti‐Rpb1‐CTD 8WG16 (Abcam, ab817) coupled to Dynabeads (Invitrogen, protein A and sheep anti‐mouse M280 IgG). For quantitative PCR, background controls were determined using uncoupled Dynabeads and enrichment was normalized to chromatin Input. Primers used for ChIP‐qPCR are listed in Table EV2.
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