Histological Analysis of Cardiac Fibrosis
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Corresponding Organization :
Other organizations : Second Hospital of Shanxi Medical University, Shanxi Medical University, Tianjin Hospital, First Hospital of Shanxi Medical University
Variable analysis
- Sacrifice method: Pentobarbital
- Fibrotic area (ratio of blue-stained area to total section area)
- Expression of TGF-β1, α-SMA, and Mac-2 (galectin-3)
- Day of sacrifice: Day 7
- Washing of hearts with heparin-containing saline solution
- Tissue fixation: 10% formalin
- Tissue embedding: Paraffin
- Tissue sectioning: 5-µm thickness
- Tissue mounting: Polylysine-coated glass slides
- Staining: Hematoxylin and Masson's trichrome
- Image capture: Microscope equipped with a camera
- Not explicitly mentioned
- Not explicitly mentioned
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