The quantitative determination of BAX and BCL-2 proteins was carried out after 24 h treatment by using Human BAX ELISA kit (DRG Instruments GmbH, Marburg, Germany) and Zymed® Bcl-2 ELISA Kit, respectively, according to the manufacturers’ instructions (Fahmy and Aldawsari, 2020 (link); Md et al., 2020 (link)). Untreated cells were considered as a control. MEL, FLV-R, or FLV-MEL nano-conjugates were used at a sub-toxic concentration (IC10).
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