Negative Staining Transmission Electron Microscopy

The images of 041 were obtained by transmission electron microscopy of the negatively-stained samples, as described in [26 (link)]. A total of 10 µL (0.2 mg/mL) of the sample solution was directly applied on the carbon-coated nickel grid (Agar Scientific, Essex, UK), and the excess liquid was drained with filter paper before staining with two successive drops of 2% uranyl acetate (pH 4.5). The prepared sample was dried and examined with a Morgagni 268(D) transmission electron microscope (FEI, Hillsboro, OR, USA).

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Labutytė G., Povilonienė S., Šimoliūnas E., Gabrielaitis D., Skapas M., Noreika A., Meškys R, & Časaitė V. (2021). Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein. Nanomaterials, 11(11), 3031.

Publication 2021

carbon-coated nickel grid Morgagni 268(D) transmission electron microscope

Agar Carbon Excess liquid Nickel Transmission electron microscope Uranyl acetate

Corresponding Organization : Vilnius University

Other organizations : Center for Physical Sciences and Technology

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Variable analysis

independent variables

Transmission electron microscopy

dependent variables

Negatively-stained samples

control variables

Sample solution (0.2 mg/mL)
Carbon-coated nickel grid
2% uranyl acetate (pH 4.5) for staining
Morgagni 268(D) transmission electron microscope

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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