Multipurpose Chinese Herbal Formulation

DHJSD comprised 6 g each of Radix glycyrrhizae, Panax ginseng, Radix achyranthis bidentatae, Eucommiae ulmoidis cortex, Poria cocos, Cortex cinnamomi, Radix paeoniae alba, Radix rehmanniae, Radix angelicae sinensis, Rhizoma chuanxiong, Herba Asari, Radix saposhnikoviae, Radix gentianae macrophyllae, Ramulus loranthi and 9 g of Radix angelicae pubescentis. The above-mentioned herbs were provided by the First Hospital of Wuhan. The specific preparation method of DHJSD has been previously reported [28 (link)]. It was formulated by the Pharmacy Department of the Wuhan First Hospital to contain 1 g/mL of crude drug. The stock solution was cooled at room temperature and stored at 4 °C prior to usage. In subsequent experiments, we diluted the DHJSD stock solution to ultimate contents of 500, 400, 300, 200 and 100 μg/mL, adopting DMEM/F12 medium with 15% fetal bovine serum (FBS).