The hematoxylin and eosin (H&E) staining and immunohistochemical analysis were performed using paraffin sections, as described previously.[27 (link)] Briefly, Mouse liver tissue was fixed overnight in 10% formalin, dehydrated, paraffin incubated, and sectioned at 5 μm. A standard H&E protocol was used for staining afterwards (95% EtOH, 70% EtOH, H2O for 30 s each, hematoxylin for 3 min, H2O, 70% EtOH, 95% EtOH for 30 s, eosin for 1 min, 95 and 100% EtOH for 30 s each, xylene for 2 min). Images were obtained with Nikon AZ 100 Macro/Zoom Microscope.