All animal procedures were done according to the Association for Research in Vision and Ophthalmology requirements and were approved by the Institutional Animal Care and Use Committee at Wayne State University (A-08-07-15) and conform to NIH guidelines. TLR4 floxed mice (B6(Cg)-Tlr4tm1.1Karp/J mice) and PDGFRα-Cre (C57BL/6-Tg(Pdgfra-cre)1Clc/J ) mice were purchased from Jackson Laboratories. TLR4 floxed mice were bred with the TLR4-PDGFRα-Cre mice to generate conditional knockout mice in which TLR4 is eliminated in retinal Müller cells. Mice of both sexes were used at 3 months of age. Genotyping and verification of knockout has been previously published (11 (link)).
Protocol detail
Retinal Müller Cell-Specific TLR4 Knockout
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Animal
Institutional animal care and use committee
Knockout mice
Mice
Pdgfrα
Retinal müller cells
Sexes
Vision
Corresponding Organization : Wayne State University
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Variable analysis
independent variables
- Genotype of mice: TLR4 floxed mice vs. TLR4-PDGFRα-Cre mice (conditional knockout mice)
- Outcome measures not explicitly mentioned
- Age of mice: 3 months
- Sex of mice: both males and females
- Positive control: Not mentioned
- Negative control: Not mentioned
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