Immunohistochemistry for Cleaved Caspase-3

Immunohistochemistry for cleaved caspase-3 was done as explained next [6 (link),17 (link)]. The sections were treated with rabbit anticleaved caspase-3 antibody (1:500; Cell Signaling Technology, Inc.) during overnight, and then incubated with biotinylated rabbit secondary antibody (1:200; Vector Laboratories) for another 1 hour. After amplification of the secondary antibody with the Vector Elite ABC kit (1:100; Vector Laboratories), 0.03% diaminobenzidine was used to visualized the antibodybiotin-avidin-peroxidase complex.

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Ko Y.J, & Ko I.G. (2020). Voluntary Wheel Running Improves Spatial Learning Memory by Suppressing Inflammation and Apoptosis via Inactivation of Nuclear Factor Kappa B in Brain Inflammation Rats. International Neurourology Journal, 24(Suppl 2), 96-103.

Publication 2020

rabbit anticleaved caspase-3 antibody biotinylated rabbit secondary antibody Vector Elite ABC kit

Antibody Avidin Caspase 3 Immunohistochemistry Peroxidase Rabbit Vector

Corresponding Organization :

Other organizations : Kangnam University, Kyung Hee University

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Variable analysis

independent variables

Treatment with rabbit anticleaved caspase-3 antibody (1:500; Cell Signaling Technology, Inc.) during overnight

dependent variables

Antibody-biotin-avidin-peroxidase complex visualization using 0.03% diaminobenzidine

control variables

Incubation with biotinylated rabbit secondary antibody (1:200; Vector Laboratories) for 1 hour
Amplification of the secondary antibody with the Vector Elite ABC kit (1:100; Vector Laboratories)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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