miRNA-directed cleavage site identification

To confirm and identify miRNA-directed cleavage sites on target genes, total RNA isolations from root and bud in A. donax were performed as formerly described^{34 (link)}. 5′ RACE System for Rapid Amplification of cDNA Ends, Version 2.0 kit (Invitrogen) was used for 5′ race experiments according to manufacturer’s instructions. The PCR products amplified using second nested primers were gel purified, cloned into pGEM-T (Promega) and sequenced. All primers used are listed in Supplementary Table 1.

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Jike W., Sablok G., Bertorelle G., Li M, & Varotto C. (2018). In silico identification and characterization of a diverse subset of conserved microRNAs in bioenergy crop Arundo donax L.. Scientific Reports, 8, 16667.

Publication 2018

5′ RACE System for Rapid Amplification of cDNA Ends, Version 2.0 kit pGEM-T

Cdna Cleavage Genes Isolations Mirna Pgem Primers Promega Root

Corresponding Organization :

Other organizations : Fondazione Edmund Mach, University of Ferrara

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Variable analysis

independent variables

RNA isolation method from root and bud in A. donax

dependent variables

Identification of miRNA-directed cleavage sites on target genes

control variables

RNA isolation method as formerly described in reference 34

controls

Positive control: Not specified
Negative control: Not specified

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