Characterization of Amyloid-Beta Carrying Extracellular Vesicles

ECVs were analyzed by NanoSight model NS300 (Malvern Instruments Company, Nanosight, Malvern, United Kingdom) as described earlier [22 (link)]. Briefly, ECV pellet samples obtained in the process of ECV isolation were resuspended in 100 μl 4% paraformaldehyde-PBS and further diluted 100 fold in PBS for analysis. Three 90 s videos were recorded for each sample. The obtained data were analyzed using Nanosight NTA 2.3 Analytical Software (Malvern Instruments Company) with the detection threshold optimized for each sample and screen gain at 10 to track the maximal number of particles with minimal background. Most of isolated ECVs carry fluorescent Aβ (1–40) cargo. In addition, Aβ is associated with ECVs of different size (see Additional file 1: Figure S1).