Chlamydia Infection Modulation Antibodies

Inhibitors against PIKfyve (YM201636) or SHIP2 (AS1949490) were purchased from Calbiochem, LY294002 (PI3 kinase) and MK2206 (Akt kinase) from Selleckchem, AG1478 (Tyrphostin #9842) from Cell Signaling. Primary antibodies against GFP (GF28R), EGFR (#PA1-1110) were purchased from Thermo Scientific, anti MyosinVb (sc-98020) from Santa Cruz, anti Myc (clone 9E10) and anti β-actin (clone AC-15) from Sigma Aldrich and Fip2 (#18136-1-AP) from Proteintech Europe. Directly FITC labeled Pathfinder antibody (#30701) against chlamydial LPS was used from Bio-rad. Antibodies against DnaK were kindly provided by S. Birkelund [62 (link)], while antibodies against Pmp21-M or Cpn0147 were generated in our lab [63 (link)]. Secondary antibodies for immunofluorescence anti rabbit/ mouse coupled to Alexa 488, 594 or 647 were purchased from Thermo Scientific. Secondary antibodies anti rabbit/ mouse/ goat coupled to alkaline phosphatase for immunoblot detection were purchased from Promega.

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Mölleken K, & Hegemann J.H. (2017). Acquisition of Rab11 and Rab11-Fip2—A novel strategy for Chlamydia pneumoniae early survival. PLoS Pathogens, 13(8), e1006556.

Publication 2017

LY294002 MK2206 anti Myc (clone 9E10) anti β-actin (clone AC-15)

Actin Ag1478 Alkaline phosphatase Anti antibodies Antibodies Antibody As1949490 Chlamydial Clone Egfr Fitc Goat Immunoblot Immunofluorescence Inhibitors Kinase Ly294002 Mk2206 Mouse Pi3 kinase Promega Rabbit Tyrphostin Ym201636

Corresponding Organization : Heinrich Heine University Düsseldorf

Top 5 similar protocols

Variable analysis

independent variables

PIKfyve (YM201636) inhibitors
SHIP2 (AS1949490) inhibitors
LY294002 (PI3 kinase)
MK2206 (Akt kinase)
AG1478 (Tyrphostin #9842)

dependent variables

Measured outcomes

control variables

Positive control: Antibodies against DnaK provided by S. Birkelund [62]
Positive control: Antibodies against Pmp21-M or Cpn0147 generated in the lab [63]
Negative control: Not explicitly mentioned

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