BALB/c (4–6 weeks old, female) and C57Bl6/J (4 to 6 weeks old, female) mice were purchased from Charles River Laboratories (C57Bl6/J: 027; BALB/c: 028). BALB/c mice were injected (s.c.) with 2 million CT26-EpCAM cells into the right flank. When the tumor size reached 2 × 2 mm, mice were randomized and i.v. treated with 2 doses of 7.5 mg/kg SUMOi or appropriate vehicle control on days 1 and 4. Mice were euthanized 24 hours after receiving the second dose. Flow cytometric analysis of the tumors was carried out as previously described (67 (link)). C57Bl6/J mice were treated with SUMOi following the same scheme. E-myc [B6.Cg-Tg(IghMyc)22Bri/J] mice were obtained from The Jackson Laboratory (stock no. 002728). Male and female mice were examined twice a week and euthanized as soon as lymph nodes were well-palpable (5 mm diameter) or any of the approved thresholds were reached.
Balb c miceCells Epcam Female Flow cytometric Lymph nodes Male Mice River Tumors
Corresponding Organization :
Other organizations :
Freie Universität Berlin, Max Delbrück Center, Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Humboldt-Universität zu Berlin, Charité - Universitätsmedizin Berlin, Goethe University Frankfurt, LMU Klinikum, German Cancer Research Center, DKFZ-ZMBH Alliance, Heidelberg University, Heidelberg Institute for Stem Cell Technology and Experimental Medicine, Helios Hospital Berlin-Buch, Universitätsmedizin Göttingen, German Knee Society, Helmholtz Zentrum München
Injections of 2 million CT26-EpCAM cells into the right flank of BALB/c mice
Treatment with 2 doses of 7.5 mg/kg SUMOi or appropriate vehicle control on days 1 and 4 in BALB/c and C57Bl6/J mice
dependent variables
Tumor size in BALB/c mice
Flow cytometric analysis of the tumors in BALB/c and C57Bl6/J mice
Lymph node size and approved thresholds in E-myc [B6.Cg-Tg(IghMyc)22Bri/J] mice
control variables
Age of mice (4-6 weeks old)
Sex of mice (female for BALB/c and C57Bl6/J, male and female for E-myc)
Supplier of mice (Charles River Laboratories for BALB/c and C57Bl6/J, The Jackson Laboratory for E-myc)
controls
Appropriate vehicle control for SUMOi treatment in BALB/c and C57Bl6/J mice
Annotations
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