HDM-Induced Asthma Model in Mice

Female C57BL/6 mice were obtained from the Experimental Animal Center of Hubei Province (Wuhan, China). The model was established by HDM sensitization and challenge. Briefly, female C57BL/6 mice received an i.p. injection of 100 μL of a solution of lyophilized HDM extract (0.1 mg/mL; Greer Laboratories) and Al(OH)₃ as an adjuvant on days 0, 7, and 14, and received 40 μL of HDM solution (3 mg/mL) or saline intranasally on days 21, 22, and 23. miR-206 agomir (5 nmol in 40 μL saline; RiboBio), control agomir, miR-206 antagomir (20 nmol in 40 μL saline), or control antagomir were administered intranasally on days 20 and 22. Twenty-four hours after the last HDM challenge, respiratory resistance in response to a range of concentrations of i.v. acetylcholine was measured using the forced oscillation technique with the FlexiVent system (SCIREQ) as previously described (73 (link)).