Selective Cytotoxicity of Stem Cell-Derived Neutrophils

Example 18

Demonstrating the Selectivity of Stem Cell Derived Neutrophils CKA for Cancer Cells

Neutrophils derived from CD34+ stem cells of three different donors were tested for CKA against both HeLa cells (cervical cancer), PANC-1 cells (pancreatic cancer) as well as non-cancer MCF-12F cells (normal breast epithelial cells).

FIG. 9 shows the maximum percentage cytotoxicity recorded by the CKA assay (xCELLigence assay—carried out for up to 45 hours) against each cancer cell type and non-cancer cell type for donors LC267, LC268 and LC269. Advantageously, SCDNs were highly selective for cancer cells showing minimal impact on non-cancer cells. Similarly to FIG. 3 showing DDN from the same donors, SCDNs from donor LC269 had the highest CKA with LC268 second, and LC267 showing the lowest CKA. Thus, it can be concluded that CKA is a genetically-defined rather than epigenetically-defined trait.

Similar results were obtained for SCDNs of donors LC252, LC253 and LC254 (FIG. 10).

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US11878033B2. Cancer-killing cells (2024-01-23). LIFT BIOSCIENCES LTD [GB]. Inventors: Alex Blyth [GB], Nico Bruyniks [GB].

Patent 2024

Assay Breast Cancer Cells Cervical cancer Cytotoxicity Donor Donors Epithelial cells Hela cells Neutrophils Pancreatic cancer Stem Stem cells

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Variable analysis

independent variables

Neutrophils derived from CD34+ stem cells of three different donors (LC267, LC268, LC269) and three additional donors (LC252, LC253, LC254)

dependent variables

Cytotoxicity (maximum percentage) against HeLa cells (cervical cancer), PANC-1 cells (pancreatic cancer), and non-cancer MCF-12F cells (normal breast epithelial cells)

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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