Culturing Human Ovarian Cancer Cell Lines

The OV2944-HM-1 (HM-1) mouse ovarian cancer cell line and JHOS2 were purchased from RIKEN BioResource Center and were cultured as previously described in ref. ^{14 (link),31 (link)}. Human ovarian cancer cell lines (OVCAR8, OVCA433, and A1847) were provided by Dr. Susan K. Murphy from Duke University. These cells were cultured and maintained in RPMI1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 g/mL streptomycin in an atmosphere containing 5% CO₂ at 37 °C. 293FT cells were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and cultured as described in ref. ^{32 (link)}. Throughout this study, we used cell lines that were passaged fewer than 30 times. All cell lines were regularly tested for mycoplasma contamination.