AAV2/5-EF1a-DIO-hChR2 (H134R)-eYFP was obtained from the UNC Vector Core (Chapel Hill, NC). The pAAV-hSyn-FLEx-mGFP-2A-Synaptophysin mRuby plasmid was a gift from Liqun Luo and was obtained from Addgene (Addgene plasmid #71760; http://n2t.net/addgene:71760 ; RRID: Addgene_71760). The pHelper and pAAV-RC plasmids used for AAV preparation were generous gifts of Ralph DiLeone. Envelope-A pseudotyped, G-deleted rabies virus expressing mCherry (RvdG-mCherry) (Osakada et al., 2011 (link)), AAV2/1-CMV-eSyn-DIO-TVA950-eYFP (1.13E+12 GC/ml) and AAV2/1- EF1α -DIO-H2B-tagBFP-Flagx3-T2Am-cB19G (1.01E+11 GC/ml) (Faget et al., 2016 (link)) were from the Salk Institute Gene Transfer Targeting and Therapeutics Core (La Jolla, CA). Sterile bacteriostatic saline, ketamine, xylazine, and meloxicam were from Patterson Veterinary Supply, Inc. (Sterling, MA). The fluorescent RetroBeads were from Lumafluor, Inc. Neurotrace fluorescent Nissl stain was from Thermo-Fisher (Waltham, MA).
Protocol detail
Viral Vector Transduction Protocol
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Gifts
Ketamine
Meloxicam
Plasmids
Rabies virus
Saline
Stain
Sterile
Synaptophysin
Therapeutics
Vector
Xylazine
Corresponding Organization : Georgia State University
Other organizations : University of Michigan–Ann Arbor
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Variable analysis
independent variables
- AAV2/5-EF1a-DIO-hChR2 (H134R)-eYFP
- PAAV-hSyn-FLEx-mGFP-2A-Synaptophysin mRuby plasmid
- Envelope-A pseudotyped, G-deleted rabies virus expressing mCherry (RvdG-mCherry)
- AAV2/1-CMV-eSyn-DIO-TVA950-eYFP
- AAV2/1-EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
- Measured outcomes not explicitly mentioned
- Sterile bacteriostatic saline
- Ketamine
- Xylazine
- Meloxicam
- Fluorescent RetroBeads
- Neurotrace fluorescent Nissl stain
- PHelper and pAAV-RC plasmids used for AAV preparation
- None mentioned
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