The surface morphology of the 3D printed scaffolds was imaged using scanning electron microscopy (SEM) to characterize the fiber morphology, roughness, and pore interconnectivity. Samples (n = 1 per formulation) were sputter-coated with 20 nm of gold (Denton Desk V, Moorestown, NJ) and SEM (FEI Quanta 400 ESEM FEG, FEICo, Hillsboro, OR) images were obtained at 2.00 kV (high voltage, HV) with 30 or 50× magnification following previous methods [38 (link)].
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SEM Analysis of 3D Printed Scaffold Morphology
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Corresponding Organization : University of Maryland, College Park
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Variable analysis
independent variables
- Formulation of 3D printed scaffolds
- Surface morphology of 3D printed scaffolds
- Fiber morphology
- Roughness
- Pore interconnectivity
- Sputter coating of samples with 20 nm of gold
- Scanning electron microscopy (SEM) imaging at 2.00 kV (high voltage, HV) with 30 or 50× magnification
- Positive control: Not mentioned
- Negative control: Not mentioned
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